Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus
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چکیده
منابع مشابه
Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression
Cotton leaf curl virus disease (CLCuD) is caused by a suite of whitefly-transmitted begomovirus species and strains, resulting in extensive losses annually in India and Pakistan. RNA-interference (RNAi) is a proven technology used for knockdown of gene expression in higher organisms and viruses. In this study, a small interfering RNA (siRNA) construct was designed to target the AC1 gene of Cott...
متن کاملCotton Leaf Curl Disease, an Emerging Whitefly Transmissible Begomovirus Complex
In recent years leaf curl disease of cotton has become a major limiting factor in the production of cotton, which is an important fiber cash crop. In the last two decades or more, leaf curl disease in cotton took on epidemic proportions in Pakistan. In the early 1990’s cotton leaf curl disease (CLCuD) appeared in the bordering parts of India in the states of Rajasthan and Punjab and spread to e...
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Cotton leaf curl disease (CLCuD) is the major biotic constraint to cotton production on the Indian subcontinent, and is caused by monopartite begomoviruses accompanied by a specific DNA satellite, Cotton leaf curl Multan betasatellite (CLCuMB). Since the breakdown of resistance against CLCuD in 2001/2002, only one virus, the "Burewala" strain of Cotton leaf curl Kokhran virus (CLCuKoV-Bur), and...
متن کاملFunctional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay
The C1 promoter expressing the AC1 gene, and V1 promoter expressing the AV1 gene are located in opposite orientations in the large intergenic region of the Cotton leaf curl Burewala virus (CLCuBuV) genome. Agro-infiltration was used to transiently express putative promoter constructs in Nicotiana tabacum and Gossypium hirsutum leaves, which was monitored by a GUS reporter gene, and revealed tha...
متن کاملIDENTIFICATION OF MOLECULAR MARKERS LINKED TO LEAF CURL VIRUS DISEASE RESISTANCE IN COTTON
The identification of molecular markers linked to leaf curl virus (CLCuV) disease resistance in cotton has the potential to improve both the efficiency and the efficacy of selection in cotton breeding programs. Genetic analysis suggested that CLCuV resistance is controlled by a single dominant gene. In this study, an interspecific F2 population derived from a cross of Gossypium barbadense and G...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2013
ISSN: 1743-422X
DOI: 10.1186/1743-422x-10-231